USA-Scientist I (Scientific) in

Scientist, Liver gene therapy mechanism of action, Genomic Medicine Unit MoA GMU contactor Basic Qualifications • Demonstrated expertise with standard aseptic cell culture technique, including experience culturing various primary cells. Experience with hepatocytes primary cell culture is highly appreciated. Experience with hepatocytes and/or liver non-parenchymal cells isolation is a plus. • Broad experience with cell-based functional assays, including transfection, cytotoxicity, cytokine release, and proliferation assays. Experience with co-culture in-vitro or MPS is a plus. • Proficiency across cellular, biochemical, and molecular techniques including protein detection (ELISA, MSD, western blot), gene profiling (RNA/DNA extraction, qPCR) and cell imaging. Experience with high content microscopy is a plus. • Broad scientific knowledge in liver biology and non-viral delivery and molecular biology. • Self-motivated with strong time management and organizational skills • Flexibility to rapidly changing priorities and deadlines • Experience in preparing high quality documents including technical reports, presentations, SOPs, and experimental protocols. • Highly collaborative with demonstrated ability to work in a matrixed team environment. • Excellent oral and written communication skills Key Responsibilities: • Maintain cultures of immortalized tumor cell lines, hepatic primary cell types (e.g. hepatocytes, LSEC and KC). • Perform hepatocytes mouse isolation from key disease models. • Optimize, design, execute, and interpret in vitro cell-based assays to characterize diverse mechanisms of gene therapies (e.g. ELISA, MSD, high content imaging) • Perform fit-for-purpose in vitro and ex vivo assays to assess phenotypic and functional read-outs to assess drug candidate toxicity and efficacy. • Prepare experimental protocols, analyze experimental data, and present study results in team and department meetings. • Document experimental methods and findings in electronic laboratory notebook POSITION OBJECTIVES Conduct and interpret cell-based in vitro assays to characterize mechanism of action related to non-viral gene therapy with focus on the hepatic environment: • Immunomodulation • Cargo cell localization • Support non-viral (LNP) oligo therapy screenings e.g. endosomal scape assessment

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